Morphogenic regulator EFG1 affects the drug susceptibilities of pathogenic Candida albicans
نویسندگان
چکیده
منابع مشابه
Squalene epoxidase encoded by ERG1 affects morphogenesis and drug susceptibilities of Candida albicans.
OBJECTIVES Functional characterization of the erg1 mutant of ergosterol biosynthesis of Candida albicans. METHODS We disrupted the ERG1 gene of C. albicans, which encodes squalene epoxidase (EC 1.14.99.7). Since the disruption of both alleles of ERG1 was lethal, the second allele of a heterozygous disruptant was placed under the control of a regulable promoter, MET3p, which is repressed by me...
متن کاملMembrane raft lipid constituents affect drug susceptibilities of Candida albicans.
By exploiting the biosynthetic pathways of raft lipid constituents, in this study we demonstrate that fluctuations in either sphingolipid or ergosterol levels result in increased drug sensitivity and morphological defects in Candida albicans cells. We show that any change in either ergosterol composition by conditionally disrupting ERG1 or in sphingolipid composition by homozygously disrupting ...
متن کاملEfg1 involved in drug resistance by regulating the expression of ERG3 in Candida albicans.
The ERG3 gene in Candida albicans was identified as a gene whose mRNA level was higher in the cph1/cph1 efg1/efg1 double mutant than in the wild-type cells. Further study showed that Efg1, but not Cph1, negatively regulated ERG3. Mutations in EFG1 consistently increased the susceptibility of the cells to antifungal agents.
متن کاملEfg1, a morphogenetic regulator in Candida albicans, is a sequence-specific DNA binding protein.
Efg1 is essential for hyphal development in the human pathogen Candida albicans under most conditions. Efg1 is related to basic helix-loop-helix regulators, and therefore most workers presume that Efg1 is a transcription factor. Here we confirm that Efg1 is a DNA binding protein that can interact specifically with the E box.
متن کاملCandida albicans exhibits enhanced alkaline and temperature induction of Efg1-regulated transcripts relative to Candida dubliniensis
Filamentous growth is an important virulence trait of the human pathogenic fungi within the genus Candida, and the greater propensity of C. albicans to form hyphae has been proposed to account for the greater virulence of this species relative to the less pathogenic species C. dubliniensis. In this meta-analysis, we compare the transcriptional response of C. dubliniensis and C. albicans to the ...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2010
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2010.00639.x